No introduction found. Please add one if you know what the request means.https://vimeo.com/265757995#t=122174&p=11192819&id=268976754 https://en.wikipedia.org/wiki/Tether_(company) https://dictionary.cambridge.org/us-eng dictionary term for dox A dox is a Greek word meaning "to investigate". It is typically used in criminal law to refer to the process of gathering information on an individual, especially through searching public records and social media (sometimes also called "doxing"). A person who is "doxed" is said to have been "search-doxted." https://www.arkcorp.us/services/education/doxing-and-privacy-breaches/ https://www.youtube.com/watch?v=6vjT16J0CmkDOX, the Drug Oxidized by Cytochrome P450, can be used to measure CYP450 enzymes (CYP450s) in living cells. The use of DOX for this purpose was developed by Stephen Gadsby and Barry Ganetzky of the University of Pennsylvania to monitor CYPs in intact cells or cell cultures. DOX is an artificial dye that contains a moiety that fluoresces at about 470 nanometers when oxidized by CYPs. The fluorescence can be used to detect the CYPs directly, or the oxidized product, doxylamine. Furthermore, fluorescent probes can act as inhibitors of P450 activity .To detect the CYP450s in living cells, one must first use an antibody against the P450s to block all but this one enzyme. This is typically done with antibodies raised against purified P450s molecules. For this purpose, DOX is an excellent probe. One can afford to use relatively high concentrations of DOX (50-100 mM) in cell cultures. As the P450s are generally not found in living cells, DOX fluorescence is not directly detected by fluorescence microscopy or flow cytometry , but is detected by the extraction of the cells into an organic solvent for HPLC analysis. The presence of CYPs in vivo can be established with spectroscopic imaging at low concentrations of the dye. https://www.sciencedaily.com/release/20181227193900. htmThe ability to assay CYPs in living cells with DOX is of great importance for pharmacology (e.g., monitoring enzyme-inhibitor interactions), toxicology, and for bioassays . Many techniques used in the regulation of drugs already exist for this purpose. However, they are typically not portable. For example, electron paramagnetic resonance (EPR) is typically used to monitor drug-drug interactions (e.g., "DRUG X-DRUG Y"). Similarly, the metabolites of CYP450s are usually monitored by LC/MS or GC/MS instruments . However, they must be extracted out of the cells prior to analysis.dox mini plc software download
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